Lammers, Elise (2022) Characterization of factors that affect the entry pathway of negatively charged liposomes into HeLa cells and their cell behaviour. Bachelor's Project, Pharmacy.
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Abstract
Liposomes are compatible for drug delivery inside the human body to specific tissues due to their small size and hydrophobic and hydrophilic behaviour. In this study the uptake pathway of negative liposomes into cervical cancer cells (HeLa cells) is studied. The aim of this research is to get a better understanding of the factors that play a role in the entry pathway of negative liposomes into cells in vivo. First, sodium azide (NaAz) was used to characterize to what extent negative liposomes are taken up via active transport into HeLa cells. Subsequently, six inhibitors were used to observe via which mechanisms these liposomes enter the cells. The negative liposomes were labeled with the fluorescent dye tetramethyl indocarbocyanine perchlorate (Dil) to analyze the data with flow cytometry. It was found that depletion of ATP levels inside the cells, the internalization of negative liposomes into cells reduced with >50%. Different factors play a role in the active transport of liposomes over the plasma membrane. These include, involvement of actin and microtubules inside cells. Also, the fission of endocytic vesicles and the Na+/H+ exchanger pump in the cell membrane of HeLa cells play an important role.
| Item Type: | Thesis (Bachelor's Project) |
|---|---|
| Supervisor name: | Salvati, A. |
| Degree programme: | Pharmacy |
| Thesis type: | Bachelor's Project |
| Language: | English |
| Date Deposited: | 24 Jun 2022 10:55 |
| Last Modified: | 24 Jun 2022 10:55 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/27365 |
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