Broek, Floris van den (2022) Characterization of the uptake mechanisms for internalization of negatively charged liposomes in HeLa cells. Bachelor's Project, Pharmacy.
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Abstract
Aim: In this report we try to give a clear view of what uptake mechanisms are involved in internalization of negatively charged liposomes in HeLa cells. Materials and methods: Liposomes were exposed to six different pharmacological inhibitors that all have a different mechanism of action when inhibiting internalization pathways. Uptake of liposomes was measured by staining them with a fluorescent dye and performing flow cytometry. Results & conclusion: The different experiments that we performed showed that macropinocytosis might be the main pathway of internalization for the negatively charged liposomes. However, all known pathways of liposomal internalization seemed to be involved in uptake. Finally, liposomal internalization depends on a lot of parameters varying from cell type to liposome characteristics making. Combination of different methods and testing of more parameters in the future will clear up how liposomes exactly are internalized into the cells.
| Item Type: | Thesis (Bachelor's Project) |
|---|---|
| Supervisor name: | Salvati, A. and Reker-Smit, C. |
| Degree programme: | Pharmacy |
| Thesis type: | Bachelor's Project |
| Language: | English |
| Date Deposited: | 27 Jun 2022 12:29 |
| Last Modified: | 27 Jun 2022 12:29 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/27384 |
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