pH sensitive nanoparticles, with nanodiamonds as a drug delivery system of diazoxide. Detection of the ROS via Diamond Magnetometry

Bosscher Navarro, Laurens (2022) pH sensitive nanoparticles, with nanodiamonds as a drug delivery system of diazoxide. Detection of the ROS via Diamond Magnetometry. Master's Internship Report, Biomedical Engineering.

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Abstract

The potential for nanodiamonds in the delivery and sustained release of anticancer drugs has been recently demonstrated due to their biocompatibility, stable structure, and highly modifiable surface chemistry. The cellular response, however, to these nanomedicines was rarely studied. Lately, fluorescent nanodiamond (FND) containing Nitrogen-Vacancy (NV) defect centres was successfully utilized to monitor the intracellular free radicals’ level by optical ways, which is also called diamond magnetometry. This study aims to investigate the cellular oxidative stress when cells are treated with nanomedicine using diamond magnetometry. Specifically, hyperbranched polyglycerol (HPG) was modified to the nanodiamond surface by polymerization to increase the stability of FND in physiological environment, and a pH-sensitive structure, carboxy-dimethylmaleic anhydride (DMA), was covalently conjugated to FND surface. FT-IR and DLS were used to characterize this nanocarrier. This pH-sensitive nanodiamond drug carrier exhibits excellent stability in the physiological environment with 135 nm diameters. We found that the modification of diazoxide on nanodiamond can recover the uptake of nanodiamond by triple negative breast cancer cell lines MDA-MB-231, which was mostly blocked by the modification by hyperbranched polyglycerol. In this project, we also examined the cellular toxicity and cellular oxidative stress of Diazoxide against MDA-MB-231. Though, there are still improvements on this pr

Item Type:	Thesis (Master's Internship Report)
Supervisor name:	Schirhagl, R. and Wu, K.
Degree programme:	Biomedical Engineering
Thesis type:	Master's Internship Report
Language:	English
Date Deposited:	11 Jul 2022 10:33
Last Modified:	11 Jul 2022 10:33
URI:	https://fse.studenttheses.ub.rug.nl/id/eprint/27715

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