Measuring oxidative stress in isolated round spermatids

Dijk, Mark van (2022) Measuring oxidative stress in isolated round spermatids. Bachelor's Research Project (period 2b), Life Science and Technology.

Preview	Text Bachelors report Mark van Dijk.pdf Download (687kB) | Preview
	Text toestemming.pdf Restricted to Registered users only Download (153kB)

Abstract

With increasing parental age there is an increase in the risk for perinatal problems. One theory for the increase in this risk is the build-up of oxidative stress. Oxidative stress is the imbalance between oxidants and antioxidants. Oxidants include reactive oxygen species (ROS), among ROS free radicals contribute to this imbalance resulting in oxidative stress. The purpose of this study was to measure oxidative stress in round spermatids. A novel technique that enables the measurement of free radicals in germ cells is diamond magnetometry. To be able to perform these measurements different techniques were used including: enzymatic disaggregation, fluorescent-activated cell sorting (FACS), and purity analysis. Testis from mice were disaggregated through enzymatic disaggregation creating a testicular cell suspension, with FACS cells were sorted and a cell suspension with 90% round spermatids was obtained. Cells were used to perform T1 measurements. The hypothesis of increasing oxidative stress with increasing age could not be proven, however, this research shows that evaluation of oxidative stress in germ cells is within the contemporary possibilities of science. With control of the biological sample and a standardized protocol a definitive conclusion can be drawn.

Item Type:	Thesis (Bachelor's Research Project (period 2b))
Supervisor name:	Schirhagl, R. and Mzyk, A.I.
Degree programme:	Life Science and Technology
Thesis type:	Bachelor's Research Project (period 2b)
Language:	English
Date Deposited:	15 Jul 2022 12:35
Last Modified:	15 Jul 2022 12:39
URI:	https://fse.studenttheses.ub.rug.nl/id/eprint/27867

Actions (login required)

View Item