Leeuwen, Loes van (2022) Oxidative stress in male germ cells, with a focus on pachytene and diplotene spermatocytes. Bachelor's Thesis, Life Science and Technology.
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Abstract
Oxidative stress is believed to play an important role in male infertility. This review goes into the role of oxidative stress on spermatogenesis, with a focus on pachytene and diplotene stages. A general overview of the spermatogenesis is given, with additional attention for the synaptonemal complex. This structure is important for differentiation between the various stages of spermatocytes. Following this, the role of oxidative stress is assessed in these various stages of spermatocytes by assessing various markers for DNA damage, DNA repair and lipid peroxidation. This study showed great heterogeneity in results among various stages, resulting in an overall inconclusive result. Lastly, ways to measure oxidative stress were mentioned including indirect and direct measurements of ROS. Indirect measurements would be: detection of lipid peroxidation, low levels of antioxidants and assessing DNA damage. Direct measurements include: chemiluminescence, flow cytometry and the novel technique of diamond magnetometry. These measuring methods are crucial in making advancements in the field of male infertility and the role of oxidative stress.
| Item Type: | Thesis (Bachelor's Thesis) |
|---|---|
| Supervisor name: | Schirhagl, R. |
| Degree programme: | Life Science and Technology |
| Thesis type: | Bachelor's Thesis |
| Language: | English |
| Date Deposited: | 15 Jul 2022 12:22 |
| Last Modified: | 15 Jul 2022 12:22 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/27893 |
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