Evidence for oxidative stress in lung tissue of mice overexpressing the enzyme Tartrate-Resistant Acid Phosphatase (TRAP) using gene expression analysis

Oosting, Silke, S (2022) Evidence for oxidative stress in lung tissue of mice overexpressing the enzyme Tartrate-Resistant Acid Phosphatase (TRAP) using gene expression analysis. Bachelor's Research Project, Pharmacy.

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Abstract

Chronic Obstructive Pulmonary Disease (COPD) and asthma are very common chronic diseases characterized by inflamed lung tissue and affect many people worldwide. There is no cure for both diseases yet. Inflammation in the lung walls can cause oxidative stress. In lung tissue of mice with induced COPD or asthma there is also an increase in TRAP values measured. TRAP is an metalloenzyme that potentially causes oxidative stress since a redox reaction can take place at its active site. Oxidants can be detoxified by activation of the Nrf2-Keap1 pathway. The RNA in lung tissue of mice overexpressing Acp5 and wildtype mice was isolated, and the gene expression profile was identified. Differentially expressed genes(DEGs) were obtained using R and pathway analysis and Gene Ontology was done using GSEA and Metascape. First, RNA quality of the samples was confirmed. 11 out of 467 significantly upregulated genes were linked to responses to oxidative stress. Aldh3b1, Btk, Rgs14, Thg1l, PLCy2, Trim30a, Acp5, Syk, Ncf1 and Ncf4 and Hvcn1 were upregulated. Unbiased analysis of the DEGs indicated enrichment of pathways related to B-cell migration, activation and proliferation. In summary, this study found that Acp5 has a link to oxygen radical formation, but no substantial evidence for the induction of oxidative stress in lung tissue was found. What we did discover was that an overexpression of Acp5 gives an enrichment in the pathways involved in B-cell proliferation, activation and survival.

Item Type:	Thesis (Bachelor's Research Project)
Supervisor name:	Melgert, B.N.
Degree programme:	Pharmacy
Thesis type:	Bachelor's Research Project
Language:	English
Date Deposited:	02 Jan 2023 11:04
Last Modified:	02 Jan 2023 11:04
URI:	https://fse.studenttheses.ub.rug.nl/id/eprint/29084

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