Antibacterial Applications of Clove Essential Oil & Its main Constituents

Kekem, Marijn van (2023) Antibacterial Applications of Clove Essential Oil & Its main Constituents. Bachelor's Research Project (period 2b), Biology.

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Abstract

Xanthomonas citri subsp. citri has become an increasingly prevalent pathogen in areas where citrus fruits are produced. This bacterium causes growths on the exterior of citrus fruits known as citrus canker. These growths make the fruit ineligible for being sold, and trees infected with it need to decontaminated according to guidelines set forth by integrated management measures. We tested the efficacy of a naturally occurring compound as a bactericidal agent, namely clove essential oil, alongside some of the most abundant compounds present in clove essential oil, namely Eugenol, Isoeugenol, and β-caryophyllene. Due to ML.2 certification restriction, E. coli was used as model organism, instead of X. citri. The oils themselves were shown to be effective at concentrations of 2.5% or higher, which is consistent with the results for isolated Eugenol. Isoeugenol was shown to be effective at a lower concentration, inhibiting growth at a concentration of 1.25%. β-caryophyllene was not shown to have any bactericidal characteristics. All effective treatments have in common that they work by disrupting membrane integrity, causing nearly every cell in a permeability assay to show up as having membrane damage after only 15 minutes of treatment with the compounds at half of the Minimum Inhibitory Concentration (MIC). Assuming that X. citri subsp. citri behaves similarly to E. coli, this shows that clove oil can be used as an alternative to the harsh pesticides currently in use.

Item Type:	Thesis (Bachelor's Research Project (period 2b))
Supervisor name:	Scheffers, D.J.
Degree programme:	Biology
Thesis type:	Bachelor's Research Project (period 2b)
Language:	English
Date Deposited:	15 Jun 2023 10:33
Last Modified:	15 Jun 2023 10:33
URI:	https://fse.studenttheses.ub.rug.nl/id/eprint/29862

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