Scheij, Josephine (2023) The effect of Interleukin 10 and IL-10-derived peptides on the inflammatory responses of macrophages. Bachelor's Research Project (period 2b), Life Science and Technology.
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Abstract
In the search for novel therapies in the treatment of liver fibrosis, interleukin-10 (IL-10) has gained particular attention due to its anti-inflammatory properties. However, when liver fibrosis is virus-induced anti-inflammatory compounds cannot be used as they suppress the immune system. The treatment of virus-induced liver fibrosis therefore requires an anti-fibrotic compound with no anti-inflammatory properties. This study analyzed the anti inflammatory effect of IL-10 and used this to test the effect of IL-10 peptide derivatives P1, P2 and P3 on RAW 264.7 cells. Cytokine effect was determined by the production of nitric oxide (NO Assay) and relative TNF-α gene expression (qPCR). Cells were pre-stimulated with LPS and a combination of LPS and IFN-γ, pre-stimulated with cytokine (IL-10, P1, P2 or P3) or co-stimulated with both LPS and cytokine. IL-10 showed to have minimal to no anti-inflammatory effect on the production of NO. P1, P2 and P3 had no effect on the production of NO and thereby do not affect inflammation through this mechanism. IL-10 did significantly reduce the relative expression of pro-inflammatory cytokine TNF-α in RAW 264.7 cells when pre-stimulated with IL-10. In contrast, P1, P2, and P3 increased relative TNF-α expression suggesting pro inflammatory properties. In summary, IL-10 exhibits anti-inflammatory properties primarily through the inhibition of pro-inflammatory cytokine gene activation while P1, P2 and P3 show no anti-inflammatory properties.
| Item Type: | Thesis (Bachelor's Research Project (period 2b)) |
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| Supervisor name: | Poelstra, K. |
| Degree programme: | Life Science and Technology |
| Thesis type: | Bachelor's Research Project (period 2b) |
| Language: | English |
| Date Deposited: | 28 Jun 2023 09:06 |
| Last Modified: | 28 Jun 2023 09:06 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/30122 |
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