Bakker, Ella (2023) Analysis of Villin Expression and Free Radical Load of Butyrate-Treated HT29 Cells. Bachelor's Thesis, Biomedical Engineering.
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Abstract
The use of diamond magnetometry for detection of free radical load is an innovative technique allowing for analysis on a single-cell level. This study examined the relationship between the expression of villin and free radical load over the course of 13 days of enterocytic differentiation. The results showed a gradual increase in villin expression during differentiation, consistent with previous studies, demonstrating the enhancing effect of butyrate on enterocytic differentiation. However, a decline in villin expression was observed after day 8, raising questions about the longer-term effects of butyrate treatment and potential sources of error in the immunostaining process. T1 values were evaluated in control and butyrate-treated groups, as well as in mixed samples, consisting of half butyrate-treated cells and half green fluorescent protein expressing control cells, in order to compare cells in the same dish. While there was no statistically significant difference in T1 values for either pure or mixed samples on individual days, the mixed samples had a significant change when evaluated over the entire differentiation period, indicating a general change in free radical load. This study provides valuable insight into the complex dynamics of enterocytic differentiation, and more precise testing is important for further understanding of the effect of butyrate treatment on free radical production, cellular differentiation, and gene expression.
| Item Type: | Thesis (Bachelor's Thesis) |
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| Supervisor name: | Schirhagl, R. and Sharma, P.K. |
| Degree programme: | Biomedical Engineering |
| Thesis type: | Bachelor's Thesis |
| Language: | English |
| Date Deposited: | 05 Jul 2023 07:08 |
| Last Modified: | 05 Jul 2023 07:08 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/30203 |
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