Hulst, Rosalie van (2024) The future of scCRISPR-seq. Master's Thesis / Essay, Biomedical Sciences.
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Abstract
This essay explores the combination of CRISPR technology and single-cell sequencing, offering a deep dive into understanding genetic changes. It discusses the background of CRISPR, which won the 2020 Nobel Prize in Chemistry. scCRISPR screens, a powerful application of CRISPR, are explained, emphasizing the integration of scRNA-seq for a more detailed analysis of cellular effects. Challenges and recent advancements in the scCRISPR field are explored, showcasing innovative studies in single-nucleotide variant analysis, spatial functional genomics, and genome-wide single-cell resolution screens. Applications across molecular and cell biology, cancer research, microbiology, medical genetics, and immunology are exemplified through noteworthy studies. The essay anticipates future possibilities, with a focus on in vivo methods, non-invasive single-cell transcriptomics, and single-cell metabolomics. The challenges and potential of these emerging areas are discussed, offering a glimpse into the future of genetics research. In conclusion, the essay highlights the crucial role of scCRISPR-seq in genetics research, covering the background, current capabilities, and future directions.
| Item Type: | Thesis (Master's Thesis / Essay) |
|---|---|
| Supervisor name: | Foijer, F. and Losito, M. |
| Degree programme: | Biomedical Sciences |
| Thesis type: | Master's Thesis / Essay |
| Language: | English |
| Date Deposited: | 15 Jan 2024 08:57 |
| Last Modified: | 15 Jan 2024 08:57 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/31819 |
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