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Potential Anti-Fibrotic Effects of Interleukin-10 on Fibroblasts

Zomers, Sophie (2024) Potential Anti-Fibrotic Effects of Interleukin-10 on Fibroblasts. Bachelor's Research Project, Pharmacy.


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Understanding the mechanisms behind the development of liver fibrosis will give new insights into the drug development. Interleukin10 (IL-10) has attracted interest as it is thought that this cytokine potentially has anti-fibrotic effects. This study analyzed the anti-fibrotic and inflammatory effects of IL-10 on NIH-3T3 cells. The nitric oxide production (NO assay) was measured after stimulation with different cytokines like PDGF-β, TGF-β, IFN-γ and IL-10. Inflammation was induced by LPS. IFN-γ showed proinflammatory effects on the production of NO. IL-10 showed varying results and was unable to induce inflammation. Furthermore, the DHPAA-assay was used to measure the collagen concentration after stimulation with TGF-β and LPS. No effects of TGF-β were visible. Lastly, qPCR was used to measure the gene expression of HAS1, HAS2 and HAS3, genes encoding for hyaluronan synthase. Hyaluronan synthase synthesizes hyaluronic acid (HA), a component of the ECM. Fibrosis is characterized by less HA and it is therefore thought that the gene expression of HAS would also be lower. The 3T3 cells were stimulated with TGF-β, IFN-γ, IL-10 and LPS. IFN-γ with LPS showed an increase in HAS gene expression. This gene expression was lower with the addition of IL-10. In summary, interleukin-10 showed anti-inflammatory effects on the expression of HAS1, 2 and 3. However, no anti-fibrotic effects had been observed.

Item Type: Thesis (Bachelor's Research Project)
Supervisor name: Poelstra, K.
Degree programme: Pharmacy
Thesis type: Bachelor's Research Project
Language: English
Date Deposited: 09 Apr 2024 08:58
Last Modified: 09 Apr 2024 08:58

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