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Potential anti-fibrotic effects of interleukin-10 on fibroblasts

Musters, Manolito (2024) Potential anti-fibrotic effects of interleukin-10 on fibroblasts. Bachelor's Research Project, Pharmacy.


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Liver fibrosis is associated with many health problems like cirrhosis and liver cancers. due to different cellular pathways is ECM formed and one of the main components are collagen, and also hyaluronic acid. In this thesis will the role of the anti-inflammatory cytokine IL-10 on liver fibrosis be researched on NIH 3T3 cells. Three assays: the NO assay which measures NO production which is a good indicator of inflammation. A collagen DHPAA assay was performed to see if collagen production is influenced by IL-10 and at last a qPCR measurement was performed to see if hyaluronic acid producing genes are upregulated under the influence of IL-10. Findings showed that Il-10 had no distinctive effect on the NO release in the cell, because on its own it being an anti-inflammatory cytokine had no response. But in combination with INF-γ which induces inflammation, it gave no distinctive decrease or increase in NO release. The collagen DHPAA assay did not give sufficient results due to the positive control not being active enough and further testing did not give usable results. qPCR measurement gave upregulation of HAS1-3 genes when influenced under INF-γ + IL-10 + LPS which could indicate that more hyaluronic acid is being produced. Further studies are necessary to support this finding.

Item Type: Thesis (Bachelor's Research Project)
Supervisor name: Poelstra, K.
Degree programme: Pharmacy
Thesis type: Bachelor's Research Project
Language: English
Date Deposited: 09 Apr 2024 09:20
Last Modified: 09 Apr 2024 09:32

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