Kruijs, G. (2024) Insulin expression by beta cells affected after exocrine damage in a zebrafish model. Bachelor's Research Project (period 2b), Biology.
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Abstract
Type 1 Diabetes (T1D) is an autoimmune disorder of the beta cells affecting insulin production. T1D has been quested for over 3000 years, but with new animal models like Zebrafish, we can give a better understanding of the mechanisms. It was found that in people with T1D the weight of the pancreas was decreased, which could be interpreted as the exocrine part also being affected in people with T1D. Our research focused on if Exocrine dysfunction has a direct effect on the beta cell insulin production. Furthermore the procedure for modelling T1D was optimised using different antibodies. We found that the only antibody staining that worked was for insulin. The model used for exocrine dysfunction are zebrafish expressing nitroreductase (NTR) on the exocrine cells, that when adding nifurpirinol (NFP) would cause exocrine dysfunction. Compared to a control group, the treated zebrafish that had exocrine dysfunction showed less insulin production from the beta cells and lower beta cell count (6.4 on average compared to 19.7 in the non treated group). These results support the idea that exocrine dysfunction may play an integral role in the cause or the maintenance of beta cell loss in T1D. Therefore, this may have impact on future research as a potential explanation for T1D beta cell loss.
| Item Type: | Thesis (Bachelor's Research Project (period 2b)) |
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| Supervisor name: | Hoogaars, W.M.H. and Giepmans, B.N.G. |
| Degree programme: | Biology |
| Thesis type: | Bachelor's Research Project (period 2b) |
| Language: | English |
| Date Deposited: | 04 Jun 2024 09:42 |
| Last Modified: | 04 Jun 2024 09:42 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/32538 |
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