Preparation and characterization of a 3D printable ink from biopolymer complexation

Morar, Rhea (2024) Preparation and characterization of a 3D printable ink from biopolymer complexation. Bachelor's Thesis, Biomedical Engineering.

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Abstract

Regenerative medicine offers solutions to problems faced through transplantation and poor repair of the human body. In order to successfully generate human organs from cells outside of the body, a suitable environment needs to be created that allows cells to proliferate. Using bioinks can allow an appropriate environment to be created that supports tissue growth. Complex coacervates have much potential as bioink candidates, they have tuneable mechanical and viscoelastic properties, can be made of biocompatible materials, and are insoluble in aqueous environments. This study used rheological testing to investigate the effect of different buffer solutions on the stiffness of a quaternized chitosan-hyaluronic acid coacervate. Mechanisms, such as ionic linkages, gelation of chitosan, and salting-out, were hypothesized to be responsible for the observed behavior. Additionally, 3D printing was carried out to find suitable parameters for a smooth print. This work has formed a foundation of research on the effect of different salts and aqueous environments on the mechanical properties of quaternized chitosan - hyaluronic acid complex coacervates. Through further research and investigation, the exact cause behind the changes in mechanical properties can be identified and utilized to influence the final properties of a 3D printable complex coacervate.

Item Type:	Thesis (Bachelor's Thesis)
Supervisor name:	Kamperman, M.M.G. and Sharma, P.K.
Degree programme:	Biomedical Engineering
Thesis type:	Bachelor's Thesis
Language:	English
Date Deposited:	02 Jul 2024 11:39
Last Modified:	04 Jul 2024 13:39
URI:	https://fse.studenttheses.ub.rug.nl/id/eprint/32933

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