Characterizing nuclear pore complex components and their role in genome maintenance and stability

Kislanski, Maxime (2024) Characterizing nuclear pore complex components and their role in genome maintenance and stability. Master's Research Project 1, Biomedical Sciences.

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Abstract

The nuclear pore complex (NPC) is one of the largest protein complexes within eukaryotic cells. Highly conserved between species, it is most well-known for its role in nucleocytoplasmic transport. However, more recent studies have illuminated its additional role in genome maintenance and stability, where defects in certain NPC components can lead to stem cell exhaustion, cancer, and aging due to compromised cellular functions. Previous investigations demonstrated how the deletions of specific nucleoporins (nups) in Saccharomyces cerevisiae led to heightened sensitivity to DNA damage. Prior investigations from our lab revealed this phenotype to be partially dependent on the background strain utilized. This project aimed to create nup KO mutants from WT strains with known genetic backgrounds. We focused on the Y complex and nuclear basket, subcomplexes of the NPC. We assessed them on growth as well as DNA damage sensitivity. Additionally, we tagged nups with GFP to study their subcellular localization following the deletion of other nups. Results demonstrated DNA damaging agents have an effect on nup KO mutants, validating the NPCs role on genome maintenance. Additionally, DNA damage sensitivity of the nup60∆ mutant varied depending on strain background. Finally, GFP-tagging nups allowed visualization of the NPC components, however unexpectedly, also displayed differences in DNA damage sensitivity between the strains.

Item Type:	Thesis (Master's Research Project 1)
Supervisor name:	Chang, M.
Degree programme:	Biomedical Sciences
Thesis type:	Master's Research Project 1
Language:	English
Date Deposited:	16 Jul 2024 11:21
Last Modified:	16 Jul 2024 11:21
URI:	https://fse.studenttheses.ub.rug.nl/id/eprint/33402

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