Ozolina, Patricija Kristiana (2024) Exploring Antioxidants: OBE100 Extract and Nanodiamond-Based Quantum Sensing for Tissue Analysis. Bachelor's Thesis, Biomedical Engineering.
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Abstract
Aging and hepatic steatosis are prevalent conditions associated with oxidative stress, for which effective treatments are urgently needed. This study investigates OBE100, an extract from Eucalyptus tereticornis leaves, for its antioxidant potential in combating free radicals in liver tissue. Precision-cut liver slices from mouse models of aging, hepatic steatosis, and control animals are used in this effort. Additionally, it explores the application of nanodiamond-based quantum sensing for assessing free radicals in tissues and aims to refine this methodology by enhancing the understanding of fluorescent nanodiamond distribution within tissues. OBE100 demonstrates robust antioxidant potential; however, its onset of action is delayed and necessitates additional dosing. In models of hepatic steatosis, OBE100 effectively restores free radical levels to their pre-steatotic state, and this effect coincides with its hypolipemic activity. Viability assays also reveal no detrimental effects of OBE100 on precision-cut liver slice health. Thus, OBE100 emerges as a promising candidate for managing oxidative stress associated with steatotic liver disease and aging—two conditions urgently requiring effective treatments. Furthermore, this research demonstrates that nanodiamond-based quantum sensing can effectively assess free radical generation in tissue slices, representing a novel achievement.
| Item Type: | Thesis (Bachelor's Thesis) |
|---|---|
| Supervisor name: | Schirhagl, R. and Reyes San Martin, C.A. |
| Degree programme: | Biomedical Engineering |
| Thesis type: | Bachelor's Thesis |
| Language: | English |
| Date Deposited: | 26 Jul 2024 14:04 |
| Last Modified: | 26 Jul 2024 14:04 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/33710 |
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