Korsten, Jesse (2025) Measuring the distribution of oligomeric states of Hsp82 by Mass Photometry. Research Project, Chemistry.
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Abstract
Molecular chaperones are an important class of regulatory proteins. A group of proteins that belong to the molecular chaperones is Hsp90. Hsp90 is a well-studied family of proteins because of their function in the conservation of cancer cells, making them a target for possible cancer therapies. Although well studied, a lot is still unknown about the mechanism of these proteins. In this project, one of the yeast orthologs of Hsp90 (Hsp82) was studied using mass photometry (MP), which is a recently developed analysis technique that can measure the mass of nanoparticles, label-free, in short measurements. In this project, MP was used to analyze the distribution between the oligomeric states of Hsp82, using different concentrations of KCl and MgCl2 and different concentrations of Poly-L-lysine coatings. In this project, it was found that when measuring Hsp82 on the MP, a large monomer population and a small dimer population were observed. It was also found that in the absence of KCl and MgCl2, no dimer state was observed, but a PLL coating did show a dimer state in the absence of these salts. Measuring on a PLL-coated slide showed an increase in dimer population compared to uncoated measurements. It was also found that in most cases, the dimeric population of Hsp82 decreased over time with varying intensities between the different buffers and concentrations of PLL coating.
| Item Type: | Thesis (Research Project) |
|---|---|
| Supervisor name: | Tych, K.M. |
| Degree programme: | Chemistry |
| Thesis type: | Research Project |
| Language: | English |
| Date Deposited: | 07 Jul 2025 13:59 |
| Last Modified: | 07 Jul 2025 13:59 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/35828 |
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