Investigating Substrate Induced Oligomeric Shifts in Phosphoglucose Isomerase (PGI)

Kelepeniotis, Gerasimos (2025) Investigating Substrate Induced Oligomeric Shifts in Phosphoglucose Isomerase (PGI). Bachelor's Thesis, Chemistry.

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Abstract

This study investigates the oligomeric behavior of phosphoglucose isomerase (PGI) from Saccharomyces cerevisiae in response to glucose-6-phosphate (G6P) using mass photometry, focusing on measurements performed on uncoated glass and 0.01% poly-L-lysine (PLL)-coated slides. On uncoated coverslips, PGI predominantly existed as a dimer (approximately 101–111 kDa) with a minor monomeric population (approximately 53–59 kDa). Across a G6P concentration range of 0 to 1 mM, the dimer fraction increased from 75% to 84%. In contrast, measurements on 0.01% PLL-coated slides showed a more stable oligomeric distribution, with the dimer consistently dominant (88–80%) and only minimal changes in the monomer proportion. No higher-order oligomeric forms were detected under any condition. These results indicate that PGI maintains a stable dimeric state upon substrate addition. Moreover, the observed differences between surface treatments highlight the influence of the electrostatic environment on enzyme–substrate interactions and the detectability of subtle structural dynamics.

Item Type:	Thesis (Bachelor's Thesis)
Supervisor name:	Tych, K.M.
Degree programme:	Chemistry
Thesis type:	Bachelor's Thesis
Language:	English
Date Deposited:	07 Jul 2025 14:10
Last Modified:	07 Jul 2025 14:10
URI:	https://fse.studenttheses.ub.rug.nl/id/eprint/35842

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