EFFECTS OF REPEATED SLEEP DEPRIVATION ON BEHAVIOR AND MEMORY IN 5XFAD MICE DURING EARLY STAGES OF ALZHEIMER’S DISEASE

Karyampa, Emmanouela Sevasti (2025) EFFECTS OF REPEATED SLEEP DEPRIVATION ON BEHAVIOR AND MEMORY IN 5XFAD MICE DURING EARLY STAGES OF ALZHEIMER’S DISEASE. Master's Research Project 2, Biomedical Sciences.

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Abstract

Sleep is critical for memory, emotional regulation, and brain health. Disruptions in sleep, especially during NREM and REM stages, are common with aging and linked to Alzheimer’s disease (AD). This study explores how repeated sleep deprivation (SD) during the preclinical stage of AD affects behavior, using 5xFAD mice. Male and female 5xFAD and WT mice underwent 0, 1, 2, or 3 rounds of SD (each 4 days, with 10-day recovery). Anxiety-like behavior was assessed using OFA, EPM, EZM, and LD Box tests, while memory was tested with the Object Location Memory (OLM) task. At baseline, no behavioral differences were found between genotypes. After one SD round, 5xFAD mice showed increased anxiety and activity in the EPM, while WT mice had milder changes. After two SD rounds, 5xFAD mice showed reduced anxiety-like behavior but increased activity, particularly in the EZM. Behavioral changes varied by test type, with some 5xFAD groups showing reduced anxiety in OFA and LD Box. In cognition, no strong memory preference was observed in OLM, but 5xFAD mice showed increased exploration after repeated SD, suggesting either hyperactivity or impaired habituation. Overall, repeated SD leads to genotype-specific behavioral changes and highlights sleep as a modifiable factor in AD progression.

Item Type:	Thesis (Master's Research Project 2)
Supervisor name:	Havekes, R.
Degree programme:	Biomedical Sciences
Thesis type:	Master's Research Project 2
Language:	English
Date Deposited:	15 Jul 2025 10:55
Last Modified:	15 Jul 2025 10:55
URI:	https://fse.studenttheses.ub.rug.nl/id/eprint/36305

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