Chen, Kaily (2025) Reversing Resistance Of Methicillin-Resistant Staphylococcus aureus By Affecting Bacterial Membrane Domains. Master's Thesis / Essay, Biomedical Sciences.
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Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is the second leading bacterium by order of deaths associated with resistance. The novel target against MRSA is the functional membrane microdomains (FMM), which deletes resistance of the bacteria against ß-lactam antibiotics, namely oxacillin, when it is dissolved by administrating AMC-109. This work is designed to further understand how AMC-109 and other FMM-dissolving-antibiotics reverse the resistance. In particular, we evaluate the stress response in bacteria by measuring the radical oxygen species (ROS) that are produced by bacteria when the antibiotics are administered, and test the susceptibility of MRSA to other antibiotics that shall dissolve the FMMs alone and in combination with oxacillin. We demonstrate that optimization of the experimental setup is required for accurate detection of ROS, especially the measuring media needs refinement to obtain reliable results. Additionally, we show that another FMM-dissolving antibiotic, N-alkylamide 3d, lowers the minimum inhibitory concentration below the susceptibility breakpoint, thus marking a successful reversal of resistance against oxacillin. The supplementation of N-alkylamide 3d could be applied with other ß-lactam antibiotics to eliminate MRSA where the re-establishment of sensitivity is expected.
| Item Type: | Thesis (Master's Thesis / Essay) |
|---|---|
| Supervisor name: | Melcrova, A. |
| Degree programme: | Biomedical Sciences |
| Thesis type: | Master's Thesis / Essay |
| Language: | English |
| Date Deposited: | 28 Jul 2025 10:59 |
| Last Modified: | 28 Jul 2025 10:59 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/36518 |
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