Cabooter, Lisa (2026) Stress-induced Senescence in S. cerevisiae. Master's Thesis / Essay, Biomedical Sciences.
|
Text
Stress-inducedSenescenceLisaCabooters6081584.pdf Download (6MB) | Preview |
Abstract
Cellular senescence is a stress response characterized by permanent proliferative arrest and the maintenance of metabolic activity. Although cellular senescence is well studied in mammalian models, the lack of cell type specificity highlights the need for a simplified model. This study examined whether environmental stress and conditional genetic defects can induce senescence-like states independent of replicative age in Saccharomyces cerevisiae. Acute heat shock generated an alive and undivided subpopulation of cells, whereas high glucose treatment led to either cell death or a recovery of proliferation. A screen of temperature-sensitive mutants revealed that alterations in several genetic pathways lead to prolonged proliferative arrest. These pathways include telomere maintenance, DNA replication, chromosome segregation, mitochondrial dysfunction, ribosome biogenesis, protein trafficking, and chromatin maintenance. These findings show that diverse forms of cellular damage can induce a common replication-independent senescence-like state in S. cerevisiae, providing a framework for studying stress-induced senescence and its conserved mechanism across eukaryotes.
| Item Type: | Thesis (Master's Thesis / Essay) |
|---|---|
| Supervisor name: | Chang, M. and Veenhoff, L.M. |
| Degree programme: | Biomedical Sciences |
| Thesis type: | Master's Thesis / Essay |
| Language: | English |
| Date Deposited: | 24 Jun 2026 12:41 |
| Last Modified: | 24 Jun 2026 12:41 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/37322 |
Actions (login required)
 |
View Item |