Baack, Fiona (2026) Molecular Insights into Polyploid Giant Cancer Cells: An Investigation of Cell Proliferation and Stress Adaptations Mechanism in High Grade Serous Ovarian Carcinoma. Master's Research Project 2, Biomolecular Sciences.
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Abstract
Ovarian cancer research increasingly focuses on polyploid giant cancer cells (PGCCs), as they present a major clinical challenge due to their ability to survive chemotherapy, remain dormant within tumors, and contribute to disease relapse. This study focused on investigating the cell proliferation and stress adaptation mechanisms of PGCCs. To investigate the underlying mechanisms, Kuramochi cells were labeled with histone-2B GFP/mCherry and FUCCI(CA)5. Exposure to CP resulted in multinucleated PGCCs, which showed asynchronous nuclear mitotic activity and mitotic alterations through multiple spindle poles. Additionally, to build on previously conducted proteomic analyses, it was investigated whether PGCCs downregulate STING as a stress adaptation mechanism to evade the immune system. Western blot results showed low STING protein levels in CP-treated PGCCs compared to untreated Kuramochi cells. Our results suggest a mechanism promoting chemotherapy resistance through buffering lethal damage or enhancing DNA repair responses.
| Item Type: | Thesis (Master's Research Project 2) |
|---|---|
| Degree programme: | Biomolecular Sciences |
| Thesis type: | Master's Research Project 2 |
| Language: | English |
| Date Deposited: | 21 Jul 2026 07:36 |
| Last Modified: | 21 Jul 2026 07:36 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/37452 |
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