Kruijer, Eva (2026) Developing a light-inducable spindle assembly checkpoint in Xenopus laevis egg extract. Master's Research Project 2, Biomolecular Sciences.
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Abstract
Faithful chromosome segregation during cell cycle progression is safeguarded by the spindle assembly checkpoint (SAC). Strikingly, in many organisms this key regulatory checkpoint is absent during early embryonic cell development. However, it is unknown why the SAC is inactive in early embryonic divisions. Here, we developed a light-inducible ectopic SAC (eSAC) activation system based on a chemical-inducible eSAC, allowing SAC activation independently of kinetochores. In this study, we provided the required constructs for the optogenetic systems, improving on previously designed light-inducible systems. These constructs were overexpressed and purified, resulting in the necessary proteins, which were functionally tested. Development of an optogenetic system can ultimately provide a non- invasive tool for rapid, reversible, kinetochore-independent activation of the SAC, allowing investigation of diffusion of the SAC signal in early embryonic development.
| Item Type: | Thesis (Master's Research Project 2) |
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| Supervisor name: | Kamenz, J.L. |
| Degree programme: | Biomolecular Sciences |
| Thesis type: | Master's Research Project 2 |
| Language: | English |
| Date Deposited: | 25 Aug 2026 13:46 |
| Last Modified: | 25 Aug 2026 13:46 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/37508 |
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