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Marine Microbial Community Fingerprinting: An Analysis between “classical” Microscopy and DGGE

Klerks, R.L. (2010) Marine Microbial Community Fingerprinting: An Analysis between “classical” Microscopy and DGGE. Bachelor's Thesis, Biology.

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The world‟s oceans contain on average 100.000 microbes per milliliter of seawater and despite their size these microbes are the biological motors that drive global and local biogeochemical cycles and processes. Community analysis has always been of importance to the scientific world and helps in understanding the mechanisms and the flow behind diversity. This literature research aims to discuss the differences and commonalities between the microscopy technique and various fingerprinting and identification techniques. The PCR-based method of DGGE is discussed in-depth due to its increasing use in the marine microbial world. Over the past 20 years a lot of research has been done to replace and/or aid the “classical” microscopy techniques due to its limitations especially in marine planktonic communities. Molecular techniques such as DGGE and clone libraries have been adapted in order to easily fingerprint communities through their 16s and 18s ribonucleic acid (RNA) and deoxyribonucleic acid (DNA). Although more and more species are discovered through their respective sequences, it is unclear whether these are different morphotypes of a single species or an actual new species. Because of these uncertainties it is important to realize that morphological information still plays a vital role in community analysis.

Item Type: Thesis (Bachelor's Thesis)
Degree programme: Biology
Thesis type: Bachelor's Thesis
Language: English
Date Deposited: 15 Feb 2018 07:31
Last Modified: 15 Feb 2018 07:31

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