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A new experimental setup for UV-exposure experiments on phytoplankton

Benjamins, S. (1998) A new experimental setup for UV-exposure experiments on phytoplankton. Master's Thesis / Essay, Biology.

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We designed a culture chamber for phytoplankton in which we attempted to imitate natural temperature and lighting conditions as strictly as possible. In this chamber, PAR doses varied from 144 μE/m2/s to 266 μE/m2/s. PAR was generated by 10 TL fluorescent lights, UVR by 4 UV-A and 1 UV-B TL fluorescent light.This combination produced a spectrum comparable to conditions in the field. In this culture chamber, six species of Antarctic phytoplankton were tested for their susceptibility to growth inhibition caused by UV-radiation. In addition, cells were tested for UV-B-induced DNA damage (specifically thymine dimers) by the immunoslot blotting technique. Results indicate that. for several species, UV-B can influence growth rates. In other species, no effect could be found; at least two species failed to grow under these circumstances. Thymine dimer detection proved more difficult than expected. Summarising, this experimental setup has proven a good addition to the equipment of this laboratory.

Item Type: Thesis (Master's Thesis / Essay)
Degree programme: Biology
Thesis type: Master's Thesis / Essay
Language: English
Date Deposited: 15 Feb 2018 07:31
Last Modified: 15 Feb 2018 07:31

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