Nijhuis, N. (1999) The effect of a synthetic peptide fragment of IL-1ß on the immune response and IL-1ß expression in vivo in rainbow trout Oncorhynchus mykiss. Master's Thesis / Essay, Biology.
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Abstract
In mammals the IL-13 peptide 163-172 is known to exert immunostimulatory effects in vivo. Therefore it may be used as an adjuvant. To find out whether a part of the IL-i 3 peptide also stimulates the immune system in teleosts a synthetic fragment of rainbow trout IL-1 was produced. The activity of this fragment was tested in vivo in rainbow trout Oncorhynchus mykiss. The effects of immunisation with different concentrations of the fragment together with a weak antigen, human gamma globulin, were monitored. In addition the effects of the IL-i ( peptide on the non-specific immune system were tested. No significantly enhanced antibody production was apparent in the immunisation experiment. Complement activity was also unaffected after peptide administration. However IL-i expression was clearly induced two days after peptide stimulation. This can be explained by the fact that IL-i is known to stimulate the expression of its own gene. The lack of effects on antibody production and complement activity may suggest the fragment may not have been powerful enough. Experiments with the whole IL-i protein may however reveal its immunostimulatory effects and is certainly worth testing in future.
| Item Type: | Thesis (Master's Thesis / Essay) |
|---|---|
| Degree programme: | Biology |
| Thesis type: | Master's Thesis / Essay |
| Language: | English |
| Date Deposited: | 15 Feb 2018 07:44 |
| Last Modified: | 15 Feb 2018 07:44 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/9407 |
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