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Towards NMR-based identification of oxygen diffusion pathways in Alditol Oxidase

Kiers, S.W. (2011) Towards NMR-based identification of oxygen diffusion pathways in Alditol Oxidase. Bachelor's Thesis, Chemistry.

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Flavin containing proteins play an important role in many cellular processes. Recent study on oxygen utilizing flavoenzymes indicated that oxygen reaches the flavin cofactor via specific ‘oxygen tunnels’. The goal of this bachelor-project is to use NMR to confirm these oxygen diffusion pathways in Alditol Oxydase (AldO). For this, triple (13C, 15N, D) labelled and unlabelled AldO was used. Unfortunately the purification of triple labelled AldO did not succeed, probably due to overheating during cell lysis. Therefore the NMR experiments had to be conducted with the unlabelled protein. By comparing the spectra of an atmospheric and a degassed sample, residues that were affected by the presence of oxygen could be identified. However, these residues did not match the ones found in previous research, which could not be distinguished because the spectra were too crowded. For this, triple labeled protein is needed.

Item Type: Thesis (Bachelor's Thesis)
Degree programme: Chemistry
Thesis type: Bachelor's Thesis
Language: English
Date Deposited: 15 Feb 2018 07:47
Last Modified: 15 Feb 2018 07:47

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