Homan, Esther (2021) Single-cell techniques to reveal clonal heterogeneity in acute myeloid leukemia. Master's Colloquium, Biomedical Sciences.
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Abstract
Acute myeloid leukemia (AML) is a heterogenous type of cancer, in which the bone marrow produces abnormal blood cells of the myeloid stem cell line. Sequencing reveals the accumulation of somatic mutations in leukemic cells. These first mutations in epigenetic factors provide an advantage to establish a founding clone. Subclones arise when additional mutations occur, that can give a competitive advantage over ancestral clones. New subclones will emerge during the progression of the disease. Many types of therapy exist for AML, however about 30% of the patients experience drug resistance or relapse. Bulk sequencing already suggests the problem of clonal heterogeneity, but it cannot elucidate clonal architecture. In this colloquium, the most current findings are discussed to give an answer to the question: how can clonal heterogeneity in AML be mapped at the cell biological level? Therefore, clonal evolution in AML is ssequenced at the single-cell level. Found is that AML tends to occur via a branched evolutionary route, clonal evolution appears in a clear order of events, several mutations do not occur in the same clone and clonal heterogeneity changes as consequence of treatment. Single-cell RNA sequencing (scRNA-seq), TARGET-sequencing, CITE-sequencing and REAP-sequencing, and single-cell DNA sequencing (scDNA-seq) are discussed based on examples.
| Item Type: | Thesis (Master's Colloquium) |
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| Supervisor name: | Schuringa, J.J. and Boom, V. van den |
| Degree programme: | Biomedical Sciences |
| Thesis type: | Master's Colloquium |
| Language: | English |
| Date Deposited: | 07 Oct 2021 06:46 |
| Last Modified: | 07 Oct 2021 06:46 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/26177 |
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