Steringa, Lisanne (2022) Untargeted lipidomics of propionic patients vs Targeted lipidomics of sphingolipids. Master's Internship Report, Pharmacy.
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Abstract
This study is focused on the two main branches of lipidomics: untargeted and targeted lipidomics. The purpose of the first part of this study is based on untargeted lipidomics using an ultra-high liquid chromatography system coupled to a quadrupole time-of-flight mass spectrometer. The aim is to explore the difference in lipid profiles of patients with propionic acidemia and healthy controls. The purpose of the second part of this study is based on targeted lipidomics using separation by high-performance liquid chromatography system coupled to a triple quadrupole mass spectrometer. The aim is the development and validates a specific and sensitive liquid chromatography triple quadrupole mass spectrometry method for quantification of sphingolipids. Results showed there was no natural grouping and differentiation between the groups regarding the lipid metabolism of PA patients and control samples. Three lipids were significantly downregulated in PA patients compared to the controls that were identified as TG(56:8), TG(44:2), and PC(20:3). An isocratic elution of 70% mobile phase B is used to elute the sphingolipid standards in the following order: lactosyl sphingosine (RT: 2.5 min) galactosyl sphingosine (RT: 2.5 min), glucosyl sphingosine (RT: 2.5 min), D-sphingosine (RT: 2.7 min) and C16 galactosylceramide (RT: 7 min).
| Item Type: | Thesis (Master's Internship Report) |
|---|---|
| Supervisor name: | Dijck-Brouwer, D.A.J. and Heiner-Fokkema, M.R. |
| Degree programme: | Pharmacy |
| Thesis type: | Master's Internship Report |
| Language: | English |
| Date Deposited: | 10 Aug 2022 12:02 |
| Last Modified: | 10 Aug 2022 12:02 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/28228 |
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