Axmann, Fabian (2024) A techno-economic feasibility study of phenylalanine production from tertiary cellulose as a building block for alkyds utilizing cellulase and Corynebacterium glutamicum. Research Project, Industrial Engineering and Management.
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Abstract
This study explores the enzymatic hydrolysis of tertiary cellulose for glucose production and its subsequent fermentation to phenylalanine using a genetically optimized Corynebacterium glutamicum strain for the production of alkyds. Results reveal that a 13% cellulase concentration efficiently achieves a 70% yield of glucose from cellulose, being lower than the anticipated 90% yield in existing literature. Glucose yields from washed and unwashed tertiary cellulose were consistent, indicating no significant impact of the cellulose purification process on the yield. Additionally, the type of buffer (phosphate or acetate) used in enzymatic hydrolysis did not significantly affect the glucose production. A C. glutamicum mutant, engineered through UV mutagenesis, showed consistent phenylalanine production across various glucose substrates derived from tertiary cellulose, suggesting that the glucose’s source—whether from pure or tertiary cellulose—does not significantly influence the phenylalanine yield. Economic analysis confirmed the feasibility of phenylalanine production from tertiary cellulose-derived glucose on production scales of 1, 5, and 8 kton/year, emphasizing the cost-effectiveness of enzymatic over acid hydrolysis. Although the production cost were found economically feasible for largescale operations, the economic attractiveness for producing alkyds from phenylalanine remains uncertain, as the target cost needs to be substantially lower.
| Item Type: | Thesis (Research Project) |
|---|---|
| Supervisor name: | Euverink, G.J.W. and Sleutels, T.H.J.A. and Krooneman, J. |
| Degree programme: | Industrial Engineering and Management |
| Thesis type: | Research Project |
| Language: | English |
| Date Deposited: | 27 Mar 2024 08:57 |
| Last Modified: | 17 Apr 2024 09:36 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/32088 |
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