Research Report 2: Comparison of different sugars for their immunostimulating capacities on influenza vaccines in vitro and in vivo

Bommel, P.E. van and Patil, H. and Huckriede, A.L.W. (2013) Research Report 2: Comparison of different sugars for their immunostimulating capacities on influenza vaccines in vitro and in vivo. Master's Thesis / Essay, Biology.

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Abstract

In this study we assessed innate immune signaling and in vivo immune responses triggered by a spray freeze-dried whole inactivated influenza vaccine. Vaccines were prepared using the stabilizer sugars inulin, dextran or a dextran/trehalose mixture. Incubation of these formulations with RAW-blue cells and a panel of receptor-specific HEK-blue cell lines revealed that inulin was able to activate NFκB through the selective interaction with toll-like receptor 2, 4 and 5. However, only low responses were seen after the addition of an endotoxin inhibitor suggesting that the observed effects were mainly caused by contamination of our samples. Immune responses following pulmonary immunization of BALB/c mice were analysed by measuring different subtypes of immunoglobulins, amounts of antibody-secreting cells and amounts of cytokine-producing CD4+ cells. None of the formulations tested induced responses that were significantly higher than those induced by liquid WIV control indicating that the strong in vitro responses that were observed from inulin do not translate into in vivo adjuvanticity. In addition, immunization with any of the formulations resulted in immune responses with a strong Th2 bias as indicated by high amounts of IL-4 secreting cells and serum IgG1. Together, these data emphasize an underlying need for further research on adjuvanted pulmonary influenza vaccines in order to develop vaccines which are more potent and give more balanced responses.

Item Type:	Thesis (Master's Thesis / Essay)
Degree programme:	Biology
Thesis type:	Master's Thesis / Essay
Language:	English
Date Deposited:	15 Feb 2018 07:52
Last Modified:	15 Feb 2018 07:52
URI:	https://fse.studenttheses.ub.rug.nl/id/eprint/10910

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