Structural and functional analyses of the mbth-homologs

Pander, S. V. (2013) Structural and functional analyses of the mbth-homologs. Bachelor's Thesis, Biology.

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Abstract

Primary and secondary metabolites are produced by microorganisms to survive in their environment, where primary metabolites are essential and mainly used for growth, the secondary metabolites are non-essential and used for a broader range of purposes. The final products can reach from antibiotics, cytostatics, fatty acids to several other compounds [1]. A small group of secondary metabolites are produced by non-ribosomal peptide synthases (NRPSs), which produce non-ribosomal proteins (NRPs). They contain several domains of which the adenylation domain, defines the specificity of the module. Adenylation domains need sometimes the assistance of a MbtH-like domain/ protein to function properly, which gives the MbtH-like protein its function as a helper protein. MbtH-like proteins are mainly produced by Actinobacteria, but they are also found in several other bacteria, generally prokaryotes [4]. The predicted structure of the MbtH-like proteins reveals the importance of two conserved tryptophan residues, which form a small pocket in the MbtH-like domain [9]. The precise function of the MbtH-like proteins is still unknown, though several depletion and reconstitution assays show, that the MbtH-like proteins help with the activation, solubilisation and overexpression of NRPSs. Also MbtH-like proteins from several species can replace each other, which means that the protein is likely a chaperone, facilitator or stabilization factor. But some NRPSs have also activity without MbtH-like proteins or can be replaced by the RubC1a domain, which is a fusion of the A-domain and a MbtH-like protein and is seen as the ancestral version of the MbtH-like protein. Because of these possibilities it is likely that MbtH-like proteins have a specific function, which could by exploited for biological applications, like strain-improvement and during the optimization of antibiotic production by secondary metabolite biosynthesis.

Item Type:	Thesis (Bachelor's Thesis)
Degree programme:	Biology
Thesis type:	Bachelor's Thesis
Language:	English
Date Deposited:	15 Feb 2018 07:55
Last Modified:	15 Feb 2018 07:55
URI:	https://fse.studenttheses.ub.rug.nl/id/eprint/11488

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