The effect of Tumor Necrosis Factor-Alpha in the development of depression following Myocardial Infarction

Bos, A. (2014) The effect of Tumor Necrosis Factor-Alpha in the development of depression following Myocardial Infarction. Master's Thesis / Essay, Biology.

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Abstract

After myocardial infarction (MI) 15-20% of the patients experience symptoms of depression. Furthermore, depression has been shown to be a risk factor for the development of myocardial infarction (MI). Inflammatory cytokines like tumor necrosis factor alpha (TNF-α) are a possible underlying mechanisms for this connection. TNF-α is elevated in patients suffering MI and the same holds true for patients with depression. TNF-α can activate two receptors, one of which leads to apoptosis and inflammation (TNF-R1), with the other leading to cellular survival (TNF-R2).We conducted this experiment to uncover if selective TNF-α interference is superior to non-selective TNF-α inhibition in diminishing depression and neuroinflammation after myocardial infarction. Mice underwent either sham surgery or the induction of MI. The MI groups were given either saline solution, Enbrel (a TNF-α blocker), a R1 antagonist or a R2 agonist. We used the effect of Enbrel as a baseline where TNF-α has no influence. The sucrose preference test, forced swim test, open field test, social interaction test and novel object recognition/special object recognition test were performed to evaluate depressive-like behavior. This was followed by IBA-1 and DCX staining to determine microglia activation and neurogenesis/neuroplasticity. We found no significant differences between the various treatment groups either in behavior or immunohistochemical stainings. Based on our results there is no indication that selective TNF-α interference is superior to non-selective TNF-α interference in the treatment of depression after a myocardial infarction. Mice that received the R2 agonist had significantly higher mortality compared to sham + saline mice.

Item Type:	Thesis (Master's Thesis / Essay)
Degree programme:	Biology
Thesis type:	Master's Thesis / Essay
Language:	English
Date Deposited:	15 Feb 2018 08:01
Last Modified:	15 Feb 2018 08:01
URI:	https://fse.studenttheses.ub.rug.nl/id/eprint/12338

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