Sollie, Thomas (2020) Using c-fos immunohistochemistry to monitor pattern separation in the dentate gyrus. Master's Research Project 1, Biomedical Sciences.
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Abstract
In order to distinguish highly similar memories, an information process called pattern separation is needed. Here, overlapping sets of information are actively pulled apart. The dentate gyrus (DG) in the hippocampus has been implicated by fMRI and ECG studies in performing this function. It achieves PS via sparse baseline activity, high firing specifici-ty and two processes called rate- and global re-mapping of cell ensembles. To this day, involve-ment has never been shown on a molecular level. Immediate early genes (IEGs) were used in this article to monitor neuronal activity in the DG fol-lowing an object location memory task, or a behav-ioural test that elicited PS. In an immunohisto-chemical cell count of IEG positive cells, no over-all increase of DG activity was found following active PS. Either PS is facilitated without overall activity increase, or the IEG method is insufficient to monitor it. Indeed, some articles report that c-fos positivity does not strongly correlate with neuronal firing, but rather represents performant path to DG input. Furthermore, one characteristic of PS is re-peated reconsolidation of similar memories. With regards to this, zif268 reveals itself as a better can-didate for IEG studies in the DG. Finally, whereas IEGs are insufficient to monitor rate remapping, they provides opportunities to study global re-mapping. While the method has obvious limita-tions, it has benefits over single cell recordings and whole brain scans.
| Item Type: | Thesis (Master's Research Project 1) |
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| Supervisor name: | Heckman, P.R.A. and Bolsius, Y.G. and Havekes, R. |
| Degree programme: | Biomedical Sciences |
| Thesis type: | Master's Research Project 1 |
| Language: | English |
| Date Deposited: | 27 Jul 2020 08:31 |
| Last Modified: | 27 Jul 2020 08:31 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/22878 |
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