Vries, Mervin, de (2022) Is eIF3h a regulator of the expression of the transcription factor C/EBPβ? Bachelor's Research Project, Life Science and Technology.
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Abstract
Multiple studies have now indicated the important role C/EBPβ, and its isoform LAP*, LAP and LIP can play in aggressive cancer types like triple negative breast cancer (TNBC). A high C/EBPβ LIP/LAP(*) isoform ratio results in more aggressive cancers and reverting this ratio has been proven to be a target for reducing proliferation and metastasis. mTORC1 can be used as a target to achieve this goal, however, this has been proven unsuccessful in TNBC. It has been observed that eIF3h is significantly upregulated in different cancers, in particular TNBC. Knowing that eIFs can influence uORF mediated isoform translation by affecting ribosome reinitiation we tested if eIF3h affects the LIP/LAP ratio in different Breast cancer cell lines. From using eIF3h knockdown (KD) and overexpression and a translational reporter we failed to come to definitive conclusion as changes in ratio were marginally in KD and overexpression had failed. Most compelling evidence was obtained from the reporter assay however experiments must be repeated for a definitive conclusion.
| Item Type: | Thesis (Bachelor's Research Project) |
|---|---|
| Supervisor name: | Muller, C. and Bruggeman, S.W.M. |
| Degree programme: | Life Science and Technology |
| Thesis type: | Bachelor's Research Project |
| Language: | English |
| Date Deposited: | 07 Jul 2022 10:24 |
| Last Modified: | 07 Jul 2022 10:24 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/27657 |
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