Lifespan of porcine iliac arteries on an ex-vivo vascular flow model

Bruin, Tessa de (2022) Lifespan of porcine iliac arteries on an ex-vivo vascular flow model. Master's Research Project 1, Biomedical Sciences.

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Abstract

Objective: This study aims to determine the lifespan of Yorkshire pig’s external iliac arteries used in an ex vivo flow model. Hence, determining the vascular functionality and endothelial denudation of perfused porcine arteries. Background: Peripheral artery disease (PAD) is a global prevalent condition. Percutaneous transluminal angioplasty (PTA) remains the therapeutic golden standard for treatment of PAD, but much of the effect of PTA on the vascular tissue is not clearly elucidated. Restenosis often occur after PTA treatments as a result of 1. elastic recoil, and 2. procedure-induced arterial or intimal hyperplasia damage. An easy in use and cost-effective ex vivo model to test the effect of these variables is lacking. Therefore, the development of an ex vivo model where porcine of even human arteries can be preserved for long periods of time would contribute to further determine the effects of PTA on the arterial wall and functionality. Methods: 46 porcine arteries were assessed in length, diameter and wall thickness before and after intervention in a PTA flow model. Eight arteries were perfused for 24 hours and eight for 48 hours, the other 30 porcine arteries served as controls. Evans Blue denuded-area staining and pharmacological vasomotor function tests were conducted after intervention, to assess the endothelium damage and endothelial depended relaxation function after perfusion. Results: the 24 hours perfused group showed a significantly lower denudation percent

Item Type:	Thesis (Master's Research Project 1)
Supervisor name:	Helfrich, W.
Degree programme:	Biomedical Sciences
Thesis type:	Master's Research Project 1
Language:	English
Date Deposited:	08 Jul 2022 12:00
Last Modified:	08 Jul 2022 12:00
URI:	https://fse.studenttheses.ub.rug.nl/id/eprint/27702

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