Smook, A.C (2022) Diamond magnetometry to measure oxidative stress in FACS-based purification of mouse spermatogonia. Bachelor's Thesis, Biomedical Engineering.
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Abstract
The age of fathers is increasing worldwide and so is the knowledge about the downside effects of advanced paternal age on fertility and child outcome. One of the factors that may play a part in this, is oxidative stress. In this experiment, the levels of oxidative stress are measured in the unipotent stem cell of the mouse germ line, spermatogonia. Oxidative stress is known to increase during aging and therefore we hypothesize that aging mouse spermatogonia show elevated levels of free radical load. Fluorescent activated cell sorting (FACS) is used to purify cell population while free radical loads are measured with a novel technique called diamond magnetometry. Diamond magnetometry focuses on the relaxometry time; a faster relaxometry time is associated with increased magnetic noise and elevated free radical load. In this experiment we successfully sorted spermatogonia with purities of at least 80 percent in all samples. Two younger populations were compared with an intermediate population and an aged population. Diamond magnetometry showed significant differences between one younger and older population (higher T1 values in younger model, P<0,001), and between the younger and intermediated population (higher T1 values in younger model, P<0,05). However, difference account for just one younger animal and the two younger population also differed statistically (P<0,001). Thus, we succeeded in sorting a purified population but to establish our hypothesis, more resear
| Item Type: | Thesis (Bachelor's Thesis) |
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| Supervisor name: | Schirhagl, R. and Elias Llumbet, A. and Mzyk, A.I. |
| Degree programme: | Biomedical Engineering |
| Thesis type: | Bachelor's Thesis |
| Language: | English |
| Date Deposited: | 15 Jul 2022 11:58 |
| Last Modified: | 15 Jul 2022 11:58 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/27885 |
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