Garcia Baucells, Pau (2022) A focus on epitranscriptomics and aging: The effect of m6A(m) modulation on C/EBPβ isoform expression. Master's Research Project 1, Biomedical Sciences.
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Abstract
The transcription factor C/EBPb gives rise to three different isoforms with antagonizing effects. The ratio of such isoforms has been proven to mediate biological processes like aging, cancer, or metabolism. Because of this, understanding the molecular mechanisms that govern or control C/EBPb’s isoform expression is relevant and necessary. One important form of regulation is the epitranscriptome, which consists of a wide range of chemical modifications in RNA that are able to determine key features of RNA biology such assplicing, stability or decay. In the present study, we aimed at elucidating the effect of m6 A(m) transcript modulation in the isoform expression of C/EBPb; adenosine decorations that confer a methylome signature controlled by methyltransferases (writers) and demethylases (erasers). To test this, we overexpressed and knocked-down the existing writers and erasers in three different cell lines and analyzed C/EBPb’s isoform expression. We report that total C/EBPb protein content is sensitive upon modulation of several players, and notably, WTAP, FTO and ALKBH5 interfere with C/EBPb’s isoform expression. In the present work we discuss whether our observations illustrate direct effects of the m6 A(m) signature of C/EBPb’s transcript and we expand the understanding of its epitranscriptome and consequent mRNA regulation: pivotal aspects for developing healthspan-aimed therapeutics.
| Item Type: | Thesis (Master's Research Project 1) |
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| Supervisor name: | Calkhoven, C.F. |
| Degree programme: | Biomedical Sciences |
| Thesis type: | Master's Research Project 1 |
| Language: | English |
| Date Deposited: | 28 Jul 2022 11:54 |
| Last Modified: | 09 Aug 2022 08:37 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/27955 |
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