Venema, Rindert (2022) In vitro studies of CD4+ T cell phenotype and function: protocol optimization. Master's Research Project 1, Biomedical Sciences.
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Abstract
Based upon preliminary data showing that the PIM-1 kinase was upregulated in CD4+ T cell subsets of active and remission GPA patients, we want to investigate the effects of Pim kinase inhibition on the viability, purity, cell proliferation and cytokine production of CD4+ T cells in vitro. Therefore, we optimized CD4+ T cell isolation, TCR receptor stimuli, the media composition, the media composition in combination with Pim inhibitors and several antibody panels to guarantee consistent and reliable results when performing in vitro studies of CD4+ T cell phenotype and function.
| Item Type: | Thesis (Master's Research Project 1) |
|---|---|
| Supervisor name: | Heeringa, P. |
| Degree programme: | Biomedical Sciences |
| Thesis type: | Master's Research Project 1 |
| Language: | English |
| Date Deposited: | 29 Jul 2022 09:27 |
| Last Modified: | 29 Jul 2022 09:27 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/28207 |
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