Investigating the possibility of using hemin as a catalyst for metabolism of drug compounds to facilitate drug development and establishment of viable drug candidates in an efficient and cost-effective manner

Nikbakht, S (2022) Investigating the possibility of using hemin as a catalyst for metabolism of drug compounds to facilitate drug development and establishment of viable drug candidates in an efficient and cost-effective manner. Bachelor's Research Project, Pharmacy.

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Abstract

In order to identify novel viable drug candidates in pre-clinical trials, new methods need to be found to make identification easier, cheaper, and more environmentally friendly, while reducing the complexity of research. This study focused on investigating whether heme can be used as a catalyst for the metabolism of substrates instead of CYP, using a simple experimental cyclic voltammetry setup in order to prevent complexity of the study. Hemin was found to be reduced at -0.361 V vs Ag/AgCl in saturated KCl. Pyridine was added to hemin, as binding of pyridine to hemin causes the molecule to become a penta-coordinated molecule, allowing the molecule to transform into a high-spin molecule after reduction, which is important for binding oxygen in its catalytic cycle. Adding pyridine caused hemin’s reduction potential to shift towards a slightly more positive potential of -0.322 V, suggesting successful binding of pyridine to hemin. Two substrates were tested to evaluate the ability of hemin to perform the hydroxylation reaction, which is a common reaction pathway performed by cytochrome P450 in phase 1 metabolism. Fenbendazole showed reactivity in voltammograms after testing, but acetyl salicylic acid did not show reactivity, thus it is suggested that hemin can act as a catalyst for metabolism, but might not be suitable for all drugs.

Item Type:	Thesis (Bachelor's Research Project)
Supervisor name:	Le, P.H.M. and Horvatovich, P.L.
Degree programme:	Pharmacy
Thesis type:	Bachelor's Research Project
Language:	English
Date Deposited:	09 Dec 2022 11:14
Last Modified:	09 Dec 2022 11:14
URI:	https://fse.studenttheses.ub.rug.nl/id/eprint/29033

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