Visser, Marije (2023) Perinatal fluoxetine exposure and myelination: are the MBP and MAG protein levels altered? Research Project 1 (minor thesis), Behavioural and Cognitive Neurosciences.
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Abstract
SSRI treatment during the perinatal period is considered to be safe for the offspring as it has no gross neuroteratogenic effects on them. Yet, previous research showed that perinatal fluoxetine exposure resulted in altered brain gene expression in juvenile rats in a.o. myelination-related genes; upregulation in the PFC and downregulation in the BLA. From this finding, along with the knowledge that myelination of the brain occurs rostrally, it is hypothesised that perinatal SSRI exposure accelerates myelination of the offspring. This study aims to look at levels of myelination-related proteins in the BLA and the PFC to verify this hypothesis. Female rats were administered either FLX or a vehicle during pregnancy and lactation. The brains of their male offspring were collected at PND 21 or 35. Fluorescent immunohistochemistry is performed for MBP and MAG. Regions of interest are the BLA and PFC. No significant differences due to perinatal exposure to FLX were found for MAG and MBP at both timepoints in the PFC and BLA. This could suggest the presence of a posttranscriptional process that compensates for the previously observed changes in RNA expression. In the FLX exposed animals as well as the control animals, the number of MAG positive cells decreased significantly over time. For MBP, no significant differences were found, but this is likely due to the type of microscope used. Ultimately, these results suggest that myelination is not accelerated between PND 21 and PND 35.
| Item Type: | Thesis (Research Project 1 (minor thesis)) |
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| Supervisor name: | Olivier, J.D.A. |
| Degree programme: | Behavioural and Cognitive Neurosciences |
| Thesis type: | Research Project 1 (minor thesis) |
| Language: | English |
| Date Deposited: | 31 Jul 2023 09:06 |
| Last Modified: | 31 Jul 2023 09:06 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/30983 |
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