Tools and Approaches for the Genetic Engineering of Basidiomycete Fungi

Jansonius, Koert (2024) Tools and Approaches for the Genetic Engineering of Basidiomycete Fungi. Bachelor's Thesis, Life Science and Technology.

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Abstract

Basidiomycete fungi are organisms of biotechnological interest because of their capabilities to produce natural products, their ability to degrade lignocellulose in e. g. wood, their nutritional value, and their potential use in building materials. The scope of available gene editing tools to study enzymatic pathways and improve production in these organisms is less advanced than for bacteria and ascomycete fungi. New developments with CRISPR-Cas have expanded possibilities for gene editing. This review discusses the development and state of the art of genetic engineering methods in basidiomycetes. The most important developments relate to development of transformation systems, selection markers (or marker-free systems), and CRISPR-Cas mediated homology directed repair (HDR). These tools are most advanced for P. ostreatus (oyster mushroom) and G. lucidum (reishi), but can be expanded to the understudied but highly relevant L. edodes (shiitake mushroom).

Item Type:	Thesis (Bachelor's Thesis)
Supervisor name:	Driessen, A.J.M.
Degree programme:	Life Science and Technology
Thesis type:	Bachelor's Thesis
Language:	English
Date Deposited:	28 Jun 2024 06:49
Last Modified:	28 Jun 2024 06:49
URI:	https://fse.studenttheses.ub.rug.nl/id/eprint/32818

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