Baartmans, Bas (2022) Overview and prospects of physical and computational antibody design. Master's Thesis / Essay, Medical Pharmaceutical Sciences.
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Abstract
Monoclonal antibodies are used as therapeutic drugs because of their predisposition for highly specific binding to macromolecular targets. Because of their therapeutic value, multiple physical approaches have been developed in the last decades for the production and isolation of strong binding antibodies. But, with the increasing computational power of the last years, the field of computational protein design has expanded greatly, and therefore questions must be asked whether these physical procedures will soon be replaced. This essay provides an overview of currently used physical and computational methods for the design of antibodies, as well as for the computational design of de novo binders. Firstly, it describes the hybridoma and display techniques and explains current methods of DNA library optimization. Secondly, state-of-the-art computational antibody and de novo protein design strategies are described. The trends and prospects of both sides will be discussed to answer the question of whether the experimental design of antibodies will become obsolete with the emergence of new computational methods.
| Item Type: | Thesis (Master's Thesis / Essay) |
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| Supervisor name: | Furst, M.J.L.J. and Poelarends, G.J. |
| Degree programme: | Medical Pharmaceutical Sciences |
| Thesis type: | Master's Thesis / Essay |
| Language: | English |
| Date Deposited: | 02 Jan 2023 10:28 |
| Last Modified: | 16 Jan 2023 12:26 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/29107 |
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